Journal: Molecular & Cellular Proteomics : MCP
Article Title: Whole Blood Proteome Dynamics Defines Predictive Diagnostic and Prognostic Signatures of Cryptococcal Infection
doi: 10.1016/j.mcpro.2025.101083
Figure Lengend Snippet: Experimental workflow and proteome overview. A , murine infection model assay with C. neoformans -infection ( purple ) and no infection (blue). Blood collection at indicated time points followed by proteomics sample preparation and measurements. B , weight distribution for each uninfected or infected mouse over the study duration (an uninfected control was matched to each endpoint collection), two-way ANOVA (mixed effects model) was applied, ∗ p -value = 0.0139. C , survival curve of C. neoformans -infected mice. D , number of peptides measured (average ± standard deviation) across five biological replicates per time point and condition. E , number of proteins measured (average ± standard deviation) across five biological replicates per time point and condition. F , number of host ( M. musculus ) and fungal ( C. neoformans ) proteins identified and quantified after valid value filtering (i.e., presence of protein across 70% biological replicates within at least one group).
Article Snippet: A comprehensive spectral library was generated using protein FASTA files against C. neoformans var. grubii serotype A (strain H99/ATCC 208821; UniProt UP000010091; 7429 sequences) and Mus musculus (17,836 Swiss-Prot reviewed entries, UniProt) ( ).
Techniques: Infection, Sample Prep, Control, Standard Deviation